FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71365686
Sequences flagged as poor quality0
Sequence length34-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1366920.19153742878615362No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG726300.10177159930894521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG74400.021.5630866
GAACGAT335250.021.17425345
CGACGAG70750.019.8441988
TCGACGA85000.018.873297
GTTCGAC76700.018.7751665
CGGTATA103250.018.36965820
ACCGCGT386900.017.662026
AGACCGC391500.017.5638924
GACCGCG387900.017.5412775
CAGACCG410650.016.8765933
CCGCGTT406900.016.7876957
CGCGTTC418450.016.3296418
AGAACGA449650.015.82674144
TCAGACC475250.015.4072472
TCACGCT284250.015.3480358
AACGCTA271500.015.12364923
ACGCTAC275000.014.98025524
TTCGGCG155850.014.97058844
AGTTCGA98950.014.9171964
CAGTTCG107700.014.7292043
Produced by FastQC (version 0.11.5)