FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71365686
Sequences flagged as poor quality0
Sequence length46-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG768980.10775206448656571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG304200.013.22525545
GGGAGGT492700.012.4495943
ATAGGGG340200.012.07657238
GTCACTC463550.012.06635958
GGAGGTC483100.012.0587344
GGCATAG334950.011.96342935
AGGTCAC508600.011.48860556
GAGGTCA527650.011.33048255
ACTCTCC503750.011.259768511
GAGCTTA359850.011.24876444
GGTCACT512750.011.0665227
AACTCTA149150.010.77136545
GTTGTCG38950.010.62879326
TGGCATA385050.010.60550234
TCAGACG143700.010.4286952
TCACTCT558250.010.176649
CATAGGG408850.010.00468537
CGTGGCG236900.09.868771
GCATAGG431050.09.49982836
CACTCTC588100.09.41908310
Produced by FastQC (version 0.11.5)