FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52171158
Sequences flagged as poor quality0
Sequence length49-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT724640.138896667771875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG63850.029.7418296
TCGACGA71600.026.6176287
CGACGAG73300.025.5979188
CGGTATA115050.025.5394320
GTTCGAC77200.024.4223165
ACGAGGA117650.022.35909710
GACCGCG175250.021.2966355
ACCGCGT178700.021.0759266
GACGAGG120900.020.9806359
GTATAGA151000.020.42601822
AGACCGC184000.020.3208794
AGTTCGA95650.020.1384414
ATAGAGT155750.019.7741924
CCGCGTT191200.019.709927
ATGACGG141400.019.69982716
CGAGGAT122850.019.60238511
CAGACCG192750.019.5278823
CAGTTCG100950.019.4632193
CGCGTTC195600.019.2665488
TATAGAG160400.019.03287323
Produced by FastQC (version 0.11.5)