FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52171158
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA157550.015.39021945
CGTAACG20050.011.99253942
ATAGGGG156150.011.94031938
CATGCAT113750.011.830829
GGCATAG155600.011.43456235
GGAGGTC236300.010.7371654
GGGAGGT243100.010.579863
TATATAC86150.010.5633257
GTCACTC225100.010.5455148
GTTGTCG29450.010.4494826
ATACATG133900.010.3724766
GGTCACT237400.010.1138517
AGCTTAG182100.010.06982645
GAGCTTA179950.010.02779544
TGGCATA180250.09.92068734
ACTCTCC247800.09.88969411
TACATGC133150.09.868457
AGGTCAC261200.09.7570426
AAACTCT262200.09.72758244
TTCCATA125450.09.7048322
Produced by FastQC (version 0.11.5)