FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73903363
Sequences flagged as poor quality0
Sequence length25-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1715560.23213557954054137No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG966810.13082083964162766No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG855290.11573086328967196No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA841920.11392174399424827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG76750.027.117346
TCGACGA87600.023.9124627
CGGTATA128850.022.08914820
CGACGAG95950.021.6672888
GAACGAT429000.020.58107445
GTTCGAC116050.018.0893215
CAGTTCG126550.017.8329223
GTATAGA175300.017.2244222
ATGACGG159800.016.60007916
ACCGCGT516000.016.447726
TCACGCT353600.016.4293928
AGACCGC526600.016.2961714
GACCGCG520600.016.2549255
CGAGGAT152300.016.01410911
ACGAGGA166950.015.9566810
ATAGAGT187750.015.92636724
AACGCTA354300.015.86962823
CCGCGTT536250.015.8222397
ACGCTAC356950.015.77702624
CAGACCG550100.015.6980983
Produced by FastQC (version 0.11.5)