FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78625168
Sequences flagged as poor quality0
Sequence length25-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1833880.23324338079633739No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG897950.11420643323776428No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG826990.10518133328503666No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA809610.10297084516245486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG44200.023.2638156
GAACGAT453100.021.34381745
TCGACGA56750.017.8809687
ACCGCGT526600.017.6079486
AGACCGC538100.017.5160454
GACCGCG530950.017.4552825
CCGCGTT544550.017.0190147
CGCGTTC545900.016.9933918
CGGTATA77250.016.89280920
AGAACGA585850.016.57219344
CAGACCG573350.016.5645663
TCAGACC624200.015.9686362
AACGCTA328500.015.66399823
ACGCTAC334450.015.37187324
CGACGAG61250.015.3182728
TCACGCT350450.015.2930988
GCGTTCT613850.015.2332589
CGCTACC342000.015.07199825
CCGGCTT380250.015.0652732
TTCACGC362450.014.9792357
Produced by FastQC (version 0.11.5)