FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78625168
Sequences flagged as poor quality0
Sequence length46-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG929440.11821151212039382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG367500.013.4637845
GGGAGGT569500.012.7379453
GGAGGTC562050.012.3462084
GGCATAG434300.011.33020935
ATAGGGG456800.011.17606138
GTTGTCG54850.010.95254226
GAGCTTA456800.010.87090644
GGTCACT632250.010.6155727
ACTCTCC629400.010.55282111
GTCACTC639850.010.5492618
TGGCATA475150.010.41767734
TCGCGCT69200.010.20992845
ATATAGC70750.09.9857846
TGTCGTT61850.09.858479528
TCAGACG164750.09.8472522
CGTGGCG276800.09.7548451
CATAGGG522250.09.60308737
TGGGAGG564450.09.4995662
CACTCTC691250.09.44910910
GAGGTCA764050.09.4145925
Produced by FastQC (version 0.11.5)