FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76503068
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA563950.010.59893494-95
ACACGTC822600.09.89239594-95
ACGTCTG688100.09.51432494-95
CACACGT995850.09.1996894-95
CACGTCT806450.09.11676294-95
ATCGGAA2632700.09.061913594-95
TCGGAAG2500300.08.92283594-95
GATCGGA2891350.08.72680394-95
TAGATCG974400.08.61991194-95
GCACACG1183150.08.61610894-95
CGGAAGA2348200.08.54410194-95
AGATCGG3241050.08.51296194-95
AGAGCAC1827550.07.778407694-95
CAGATCG1160500.07.59948694-95
AAGATCG956700.07.531633494-95
AGCACAC1524800.07.417505394-95
GAGCACA1769000.07.33503694-95
AAGAGCA2233250.07.15619494-95
GAAGAGC2492350.07.1335794-95
GTCTGAA803500.06.86983594-95
Produced by FastQC (version 0.11.5)