FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77928866
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA675800.010.78198394-95
ACGTCTG826250.09.78304594-95
ACACGTC992200.09.60156894-95
CACGTCT973200.09.09192594-95
CACACGT1189350.08.83525794-95
ATCGGAA2963900.08.67454294-95
GATCGGA3267550.08.55137194-95
TCGGAAG2843250.08.49435294-95
AGATCGG3647500.08.35946394-95
CGGAAGA2693500.08.23564994-95
GCACACG1438750.08.09427694-95
GTCTGAA854350.07.89002894-95
AGAGCAC2075900.07.825245494-95
TCTGAAC781000.07.721734594-95
TAGATCG1017100.07.714597794-95
CAGATCG1369150.07.46413994-95
AGCACAC1740600.07.40376694-95
GAGCACA2002600.07.354847494-95
AAGATCG1018350.07.285569794-95
AAGAGCA2456500.07.181629794-95
Produced by FastQC (version 0.11.5)