FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80452570
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA655650.010.26072894-95
ACACGTC958750.09.46931194-95
ACGTCTG802750.09.35683494-95
CACGTCT933900.08.94756994-95
ATCGGAA2979600.08.82306194-95
CACACGT1148750.08.73755694-95
GATCGGA3274250.08.58344794-95
GCACACG1371850.08.4740894-95
TCGGAAG2837050.08.44480494-95
AGATCGG3643750.08.26875894-95
TAGATCG1033600.08.17469994-95
CGGAAGA2667250.08.00912594-95
AGCACAC1733950.07.734472394-95
AGAGCAC2059550.07.729735494-95
AAGATCG1128000.07.57839694-95
CAGATCG1331800.07.54755794-95
GAGCACA1986450.07.310157394-95
TCTGAAC819700.07.17641794-95
GTCTGAA886750.07.067494494-95
AAGAGCA2487450.07.02317694-95
Produced by FastQC (version 0.11.5)