FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59325593
Sequences flagged as poor quality0
Sequence length48-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52750.026.8296596
CGGTATA88600.022.59889820
CGACGAG63100.022.2121968
TCGACGA63800.022.1095397
GTTCGAC66850.020.9351445
ACCGCGT106800.017.275466
AGACCGC109300.017.086184
AGTTCGA85550.016.963944
GACCGCG108800.016.9165345
GTATAGA129000.016.60268222
CAGTTCG91650.016.3504163
ACGCGTC24800.016.3340140
AACGCTA162550.016.31765723
ATAGAGT131300.016.29471624
ACGCTAC164150.016.19972224
ACGAGGA118500.016.08038310
GTTCCTA98200.015.89934915
ATGACGG121450.015.76388916
CGAGGAT112900.015.66245911
CCGCGTT117550.015.65537557
Produced by FastQC (version 0.11.5)