FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80115238
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1441850.09.605041594-95
GATCGGA1579850.09.59459894-95
AGATCGG1809100.09.41265594-95
TAGATCG564950.09.14638294-95
ACACGTC453300.09.03593594-95
TCGGAAG1403250.08.98336794-95
CGGAAGA1346650.08.75899894-95
CGTCTGA323050.08.41432594-95
CACACGT578450.07.977237794-95
CAGATCG703200.07.934744494-95
ACGTCTG409050.07.92670894-95
CACGTCT478400.07.61585794-95
AAGATCG604950.07.523627394-95
GCACACG692400.07.44212594-95
AGAGCAC1200900.06.642647394-95
GAGATCG644700.06.402198394-95
AGCACAC1025000.06.37417994-95
GAGCACA1197800.06.10744394-95
GAAGAGC1698600.05.789026394-95
AAGAGCA1583450.05.571462694-95
Produced by FastQC (version 0.11.5)