FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78263523
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1774400.09.43332394-95
GATCGGA1967850.09.38954594-95
AGATCGG2236300.09.13918994-95
TCGGAAG1751800.08.929543594-95
ACACGTC584350.08.88205594-95
CGTCTGA414200.08.83416794-95
TAGATCG634500.08.76626594-95
CGGAAGA1673950.08.44221194-95
CACACGT737350.08.00494294-95
ACGTCTG513400.07.88542894-95
CACGTCT605500.07.84320394-95
CAGATCG907650.07.81820894-95
GCACACG913350.07.652607494-95
AAGATCG661650.07.247328894-95
AGAGCAC1399850.06.96842394-95
AGCACAC1196100.06.475190694-95
GAGATCG780650.06.40481794-95
AAGAGCA1728300.06.316485494-95
GAGCACA1396400.06.26498194-95
GAAGAGC1963800.06.05676594-95
Produced by FastQC (version 0.11.5)