FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53075594
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA553240.10423623332411504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA222350.021.24545945
CGGTATA30150.018.13247720
CATGCAT156350.017.3245949
TATATAC114950.016.4206357
ATACATG182200.014.8050616
AAACTCT333050.014.58921544
TACATGC187400.014.2260957
ACGGTAT38000.014.14980519
AAAACTC351600.014.11390843
TTCCATA163300.014.1102192
GACGGTA38900.013.995934518
ATATATA146700.013.7716396
TCCATAT166700.013.6466663
TGCATTC195150.013.6033911
CCATATA139050.013.3663714
GTTGTCG46250.013.13438826
TCGACGA30700.013.11751657
GGGAGGT279350.012.8637393
ACATGCA220500.012.8251378
ATAGGGG215100.012.783710538
Produced by FastQC (version 0.11.5)