FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58352435
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA31900.019.46513420
AACTCTA178700.018.04752745
TCGACGA40600.014.4073247
GACGGTA44300.014.11812718
CATGCAT140750.014.0179649
ACGGTAT44050.013.89181419
TTCGACG41100.013.6847476
ATATAGC61600.012.5271436
TATATAC100850.012.4924657
GTTGTCG48500.012.47861126
ATAGGGG200450.012.16856238
GGGAGGT287900.012.0049813
GGAGGTC275000.011.8070824
GGCATAG199150.011.68269135
AGCTTAG206900.011.32364745
TGCATTC171050.011.31123211
GTCACTC271600.011.2984378
AGGTCAC297350.011.1750946
AAACTCT303200.011.141561544
GGTATAG57050.011.12071721
Produced by FastQC (version 0.11.5)