FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78784762
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC582400.09.65751594-95
GATCGGA2043450.09.44005894-95
ATCGGAA1855950.09.39545794-95
CGTCTGA397650.09.13057894-95
AGATCGG2320650.09.09968594-95
TAGATCG686900.09.00083694-95
TCGGAAG1790800.08.84884894-95
ACGTCTG509900.08.78503694-95
CGGAAGA1711500.08.69571994-95
CACGTCT600600.08.63674994-95
CACACGT716000.08.47027594-95
GCACACG862550.08.05846994-95
AAGATCG734850.08.01417994-95
CAGATCG888150.07.95900594-95
AGAGCAC1415550.07.420386394-95
AGCACAC1195650.06.953984394-95
GAGCACA1404800.06.81567394-95
AAGAGCA1800250.06.465959594-95
GAAGAGC1994750.06.38067494-95
GAGATCG816850.06.360852794-95
Produced by FastQC (version 0.11.5)