FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76351305
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA412150.09.5453294-95
ACACGTC597550.09.18319994-95
GATCGGA1977150.09.06196694-95
ATCGGAA1792000.09.05853594-95
AGATCGG2237250.08.88181994-95
TAGATCG666950.08.68472594-95
TCGGAAG1751000.08.68366194-95
ACGTCTG516750.08.59079794-95
CGGAAGA1691000.08.24660994-95
CACGTCT610950.08.197659594-95
CACACGT744500.08.01017994-95
CAGATCG840250.07.895581294-95
GCACACG899000.07.702561494-95
AAGATCG721850.07.412843794-95
AGAGCAC1405700.07.08034894-95
AGCACAC1205650.06.824723294-95
AAGAGCA1738650.06.53103694-95
GAGCACA1397200.06.504174794-95
GAAGAGC1977500.06.336820694-95
GAGATCG775500.06.315965794-95
Produced by FastQC (version 0.11.5)