FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76370855
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1117150.011.26089494-95
ACGTCTG1322900.010.33392794-95
ACACGTC1626350.010.09835894-95
CACGTCT1524750.09.85620994-95
CACACGT1887050.09.41955394-95
TCTGAAC1129450.09.20697894-95
GTCTGAA1255550.09.14170194-95
TCGTATG64050.09.08281912-13
GCACACG2194200.08.84127794-95
AATCTCG134700.08.4271048
CGGAAGA4270450.08.39604994-95
AGCACAC2580900.08.30217594-95
CTGAACT1140350.08.26685194-95
AGAGCAC3117400.08.17045894-95
TCGGAAG4569450.08.13040894-95
GAGCACA2937550.07.96763494-95
ATCGGAA4858450.07.92260794-95
CTCGTAT73400.07.861374410-11
GATCGGA5308300.07.7692394-95
TCCAGTC719600.07.747421794-95
Produced by FastQC (version 0.11.5)