FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80850570
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1576500.09.314568594-95
GATCGGA1741650.09.23169394-95
AGATCGG1989700.09.09163594-95
TCGGAAG1560050.08.83133894-95
TAGATCG603250.08.81545294-95
ACACGTC527300.08.79362794-95
CGTCTGA370750.08.75472894-95
CGGAAGA1519250.08.36843494-95
CACACGT670900.07.835835594-95
ACGTCTG474100.07.68982694-95
CACGTCT560700.07.525521894-95
CAGATCG788450.07.524012694-95
AAGATCG661000.07.431458594-95
GCACACG816700.07.35956394-95
AGAGCAC1294150.06.83000594-95
GAGATCG704000.06.405348394-95
AGCACAC1138000.06.157718794-95
AAGAGCA1645750.06.103381694-95
GAGCACA1327450.06.08231394-95
GAAGAGC1864050.05.881452694-95
Produced by FastQC (version 0.11.5)