FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78007645
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA435150.09.62643694-95
ATCGGAA1951450.09.29297694-95
ACACGTC640550.09.29146294-95
GATCGGA2153900.09.160294-95
AGATCGG2432950.08.72379594-95
ACGTCTG549950.08.60841794-95
TCGGAAG1905100.08.58927294-95
CGGAAGA1812450.08.43780894-95
TAGATCG705900.08.42720194-95
CACACGT792150.08.38005894-95
CACGTCT654900.08.34339694-95
CAGATCG986400.07.557485694-95
GCACACG964650.07.51255794-95
AAGATCG683150.07.318471494-95
AGAGCAC1493600.07.191121694-95
AGCACAC1264650.06.77522994-95
GAGCACA1478100.06.638052594-95
GAGATCG834150.06.44673294-95
AAGAGCA1836350.06.419198594-95
GAAGAGC2075750.06.29872694-95
Produced by FastQC (version 0.11.5)