FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80260372
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1647100.09.40694894-95
GATCGGA1812850.09.35176394-95
AGATCGG2060300.09.00678994-95
ACACGTC547500.08.79071394-95
TCGGAAG1632000.08.75008794-95
TAGATCG587000.08.58235994-95
CGTCTGA387150.08.55840194-95
CGGAAGA1570300.08.13528994-95
ACGTCTG484450.08.00022494-95
CACACGT680750.07.959595794-95
CAGATCG844900.07.81254294-95
AAGATCG633100.07.561146394-95
CACGTCT573600.07.470704694-95
GCACACG841150.07.42911994-95
AGAGCAC1342050.06.81579294-95
AGCACAC1149150.06.37646394-95
GAGCACA1341100.06.17339394-95
GAGATCG734350.06.12202294-95
GAAGAGC1891550.05.925822394-95
AAGAGCA1684800.05.923864494-95
Produced by FastQC (version 0.11.5)