FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59743873
Sequences flagged as poor quality0
Sequence length31-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1279780.21421108738631658No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG760560.12730343076351947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37600.024.11646
GAACGAT324200.020.45998845
TCGACGA47050.019.3199397
CGGTATA63200.019.18829520
GTTCGAC49500.018.3642835
ACCGCGT359150.017.5920526
AGACCGC365800.017.5546154
GACCGCG360750.017.4767075
CGACGAG53850.017.1309788
CGCGTTC374000.016.8329458
CCGCGTT375750.016.8024527
CAGACCG390850.016.5506213
AGAACGA414300.016.01024444
AACGCTA266250.015.97961223
TCAGACC429950.015.9776742
CCGGCTT299900.015.9661382
ACGCTAC268400.015.8851424
TCACGCT285050.015.652578
TTCACGC285250.015.6258197
CGCTACC277800.015.33953125
Produced by FastQC (version 0.11.5)