FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59743873
Sequences flagged as poor quality0
Sequence length46-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG797280.13344966771739086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG290900.014.1934845
GGGAGGT443050.013.392163
GTTGTCG41550.013.15869626
GGAGGTC441300.012.9352674
GGCATAG337200.012.40419935
ATAGGGG348950.012.08988738
GAGCTTA347900.011.97801644
ATATAGC54750.011.7943686
TGTCGTT49350.011.35245628
TGGCATA371300.011.33166134
ACTCTCC496950.011.04724511
GGTCACT521250.010.6228847
TGGGAGG429050.010.5990592
GTCACTC525900.010.5032868
TATAGCT60050.010.4911217
CATAGGG410450.010.25090537
GCTAAGT56450.010.20359714
CACTCTC538350.010.11828610
GAGGTCA589500.09.9924635
GCATAGG427000.09.911530536
Produced by FastQC (version 0.11.5)