FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134822977
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA545650.010.32054670
AGATCGG683850.010.30509970
ATCGGAA485650.09.89270370
TCCGATC125400.09.3757226
TAGATCG225100.08.81133870
TCGTATG82650.08.04428745
TTCCGAT153400.07.8924755
CGTATGC95700.07.20345646
TCGGAAG649200.06.60159570
TATGCCG106050.06.59941648
CCGATCT183800.06.51094537
TATCTCG113000.06.41013441
CTCGTAT110250.06.062153344
AAGATCG398500.05.88299870
CAGATCG385000.05.6705970
CGGAAGA635350.05.559662370
Produced by FastQC (version 0.11.5)