FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134822977
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG727550.09.62602670
GATCGGA593800.09.55332770
ATCGGAA525300.09.32588870
CGCCGTA71550.08.99743258
TAGATCG233250.08.64730470
GCCGTAT84700.07.765791459
CGTATCA86000.07.689036461
TTAACCG91550.07.414555532
TAACCGT89950.07.196305333
ACCGTCG94250.06.867789735
TCGGAAG751650.05.762804570
AAGATCG463850.05.4505470
AACCGTC131350.05.274391734
CCGTATC129500.05.13324660
CAGATCG401900.05.070897670
CGGAAGA724350.05.05665270
Produced by FastQC (version 0.11.5)