FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82955240
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1941250.09.73093394-95
ATCGGAA1757750.09.69446494-95
AGATCGG2211300.09.37905394-95
TAGATCG653750.09.04973394-95
TCGGAAG1708700.09.00602494-95
ACACGTC561450.08.96425694-95
CGTCTGA386100.08.95025694-95
CGGAAGA1646150.08.68274194-95
CACACGT698150.08.35096994-95
ACGTCTG494750.08.068665594-95
CAGATCG881200.07.95055194-95
CACGTCT581150.07.836290494-95
GCACACG848350.07.572185594-95
AAGATCG707600.07.55856394-95
AGAGCAC1379000.06.99584294-95
GAGATCG745900.06.47455294-95
AGCACAC1176100.06.27398994-95
AAGAGCA1771400.06.26692994-95
GAGCACA1366300.06.2191994-95
GAAGAGC1972850.06.156127594-95
Produced by FastQC (version 0.11.5)