FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82807460
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA896100.011.07587894-95
ACACGTC1309600.09.89891994-95
ACGTCTG1094750.09.83193394-95
CACGTCT1262500.09.26292194-95
CACACGT1556500.09.25927394-95
GTCTGAA1068650.08.61657794-95
ATCGGAA4023100.08.56165294-95
TCTGAAC964050.08.55584894-95
GCACACG1871200.08.49773894-95
TCGGAAG3812400.08.4236194-95
GATCGGA4420200.08.36610794-95
CGGAAGA3579650.08.34296894-95
AGAGCAC2674200.08.0888194-95
AGATCGG4897400.08.046205594-95
AGCACAC2226350.08.02260394-95
GAGCACA2537100.07.834223394-95
TAGATCG1325200.07.823294694-95
AAGAGCA3140600.07.655308294-95
GAAGAGC3588950.07.41429894-95
CTGAACT1003750.07.336083494-95
Produced by FastQC (version 0.11.5)