FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45244520
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA28800.020.16703258
GCCGTAT32350.018.06217459
CGTATCA34900.016.64207861
CCGTATC46000.013.31077160
GCGTCGT51900.011.12330511
TCGTGTA52400.011.08393714
TCGCCGT55750.010.54364557
CGTCGTG62200.09.50634612
AGCGTCG63350.09.49935910
TGGTCGC65350.09.20869754
GTCGTGT63900.09.143929513
GTCGCCG67400.08.82500456
TAGATCG47600.08.08560670
GGTCGCC77900.07.90509955
CGTGTAG75900.07.790443415
GATCGGA116400.07.785269370
GTGGTCG86900.07.287425553
AGATCGG136950.07.076925370
AGAGCGT100050.06.1545948
ATCGGAA113750.06.15184670
Produced by FastQC (version 0.11.5)