FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56780741
Sequences flagged as poor quality0
Sequence length34-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT757620.13342904418947263No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG621100.10938568061307971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52850.026.0109066
TCGACGA60700.022.8323867
CGACGAG61650.021.6411788
CGGTATA84500.021.4865820
ACCGCGT185650.020.7960856
GACCGCG187400.020.5790165
GTTCGAC66750.020.4607145
AGACCGC194600.020.1415674
CCGCGTT197900.019.5088087
CAGACCG207800.018.9054493
CGCGTTC206800.018.7018538
CAGTTCG87100.016.97213
AACGACG40950.016.81190116
GAACGAT240350.016.58534845
AACGCTA232450.016.44428823
ACGCTAC233300.016.36508824
AGTTCGA86500.016.3614774
TCACGCT246050.016.2671648
TCAGACC257050.016.1945342
TTCACGC256950.015.9360997
Produced by FastQC (version 0.11.5)