FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55356901
Sequences flagged as poor quality0
Sequence length50-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG3525610.6368871696773633No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT1265970.2286923540029815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1033950.18677888055908332No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG931890.16834215484714363No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG708530.1279930753349072No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA693630.12530145067188642No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT583340.10537800878701645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG225750.033.248636
GTTCGAC230150.032.380085
TCGACGA235100.032.0315937
CGACGAG233300.031.8061718
AGTTCGA238650.031.3210874
CAGTTCG250000.029.881163
GCCGGCT656100.029.428921
CGAGGAT307550.028.67071311
GACGAGG332600.028.0334229
ACGAGGA333900.028.01866710
CCGGCTT760850.027.4001452
CGGTATA380700.027.35175320
GTATAGA428850.026.15377822
TATAGAG433850.025.73308423
ATAGAGT431050.025.7279924
TTCACGC849250.024.6972737
TAGAGTT452050.024.48302525
TCACGCT863250.024.252438
TCAACGA107850.024.0747158
GGTATAG441950.023.5814321
Produced by FastQC (version 0.11.5)