FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53897954
Sequences flagged as poor quality0
Sequence length50-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1634680.30329166112687694No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT1283070.2380554185785976No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1004930.1864504912375709No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA706880.13115154612362465No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCT585550.10864048754058457No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG552830.10256975617293376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG224450.033.9324156
GTTCGAC230350.032.9087565
TCGACGA233100.032.6345947
CGACGAG231150.032.286948
AGTTCGA244100.031.0458094
CGAGGAT285650.030.7900211
CAGTTCG250950.030.5300963
GACGAGG313950.029.5626149
ACGAGGA318750.029.32922410
CGGTATA371450.029.07468220
GTATAGA420900.026.46598622
ATAGAGT426750.026.33516724
TATAGAG433850.026.01828423
ATGACGG399700.025.61811616
TAGAGTT440700.025.52707725
GCCGGCT388800.024.3531651
GGTATAG444850.024.02448821
TGACGGT430850.023.71893317
CCGGCTT448000.023.6865442
GATGACG423300.023.49376115
Produced by FastQC (version 0.11.5)