FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53897954
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA929150.17239058833290777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA877010.16271675173421238No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG838380.15554950379006965No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG681710.12648161004404732No Hit
CTTCCATATATACATTCCTTGGAAAGCTGAACAAAATGAGTGAAAACTCTA610970.11335680757009813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA341000.025.14354145
CATGCAT211300.021.977299
AAACTCT435750.019.88267144
ATACATG249600.018.7489556
TGCATTC250650.018.34749211
TATATAC177150.018.30137
AAAACTC486900.018.09945943
TACATGC259750.017.9124137
TTCCATA221900.017.7840462
ATATATA204400.016.752986
TCCATAT233100.016.697853
CCATATA191450.016.5111624
TTATACA149050.016.3777584
ACATGCA296550.016.1449248
TTTATAC149950.016.1144143
ATATACA221500.016.1098618
GGGAGGT391250.016.0207733
GGAGGTC376800.015.9723794
GTTGTCG51250.015.8040726
CATATAT212900.015.2598495
Produced by FastQC (version 0.11.5)