FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59504405
Sequences flagged as poor quality0
Sequence length31-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3311380.5564932545750184No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1439420.24190141889495406No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1388380.2333239026589712No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC836610.14059631383592525No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG661990.11125058724643999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT624650.027.25689945
TTCGACG38450.022.2955256
AGAACGA821500.020.75820444
AGACCGC892650.018.7605554
GACCGCG886700.018.6128185
ACCGCGT890550.018.560166
CAGACCG907450.018.5216053
CCGCGTT909350.018.1686557
CGCGTTC922150.017.9213458
TCAGACC978450.017.845122
AAGAACG966700.017.62399343
TCGACGA48850.017.5945877
GCGTTCT961300.017.2265859
CTCAGAC1048300.017.1497421
ATACGGA1027350.016.3647933
AATACGG1029050.016.35736532
CGACGAG50850.016.327358
TACGGAG1029550.016.15955734
CGTTCTC1068750.016.14728710
CAATACG1060700.016.03037831
Produced by FastQC (version 0.11.5)