FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63791284
Sequences flagged as poor quality0
Sequence length50-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1333210.20899563645716868No Hit
GAAAAAAATTCCAAAAGAGAACCACACACTACCATATCCAAACAACTTTTG872630.1367945501771057No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA809180.12684805027595933No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT794750.12458598575943385No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTAT668470.1047901779183501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG147300.033.1004526
CGACGAG144800.032.382248
GTTCGAC155450.032.1617165
TCGACGA154050.031.723127
AGTTCGA171200.029.3869114
CGGTATA248500.028.6204120
ACGAGGA227800.028.37662110
CAGTTCG181350.027.878633
GACGAGG227550.027.6266569
CGAGGAT224350.026.6567811
GCCGGCT310800.025.8453031
TATAGAG301750.025.30709623
ATGACGG266400.025.28688416
GTATAGA307700.024.93472922
ATAGAGT308100.024.89505424
CGTTACC49250.024.677545
TGACGGT280800.024.04621117
CCGGCTT368650.023.645032
AACGACG120600.023.39526616
TCGTTAC54400.022.91342444
Produced by FastQC (version 0.11.5)