FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80601503
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1987700.09.46740194-95
ATCGGAA1799650.09.43358194-95
AGATCGG2256200.09.03036294-95
CGTCTGA399400.08.90965594-95
ACACGTC582250.08.858294-95
TCGGAAG1755600.08.8578694-95
CGGAAGA1685800.08.46914594-95
TAGATCG698700.08.46230394-95
CACACGT727150.08.2342494-95
ACGTCTG506950.08.2073794-95
CAGATCG875250.07.89439894-95
CACGTCT596350.07.86602194-95
GCACACG873100.07.72572794-95
AAGATCG694500.07.459666394-95
AGAGCAC1396150.07.064423694-95
AGCACAC1207550.06.71461294-95
GAGCACA1406550.06.411964494-95
AAGAGCA1782300.06.38907694-95
GAGATCG750200.06.33352694-95
GAAGAGC1998500.06.190986694-95
Produced by FastQC (version 0.11.5)