FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63547246
Sequences flagged as poor quality0
Sequence length31-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1442470.22699174091667168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG59700.025.5905916
GAACGAT312800.023.4426845
TCGACGA67600.022.599697
CGACGAG68500.021.8100628
CGGTATA92600.021.06571820
GTTCGAC81050.018.8218485
GACCGCG372850.018.7737675
AGACCGC380000.018.6629664
ACCGCGT377200.018.6288136
CAGACCG390350.018.3066883
CCGCGTT392000.017.9252537
CGCGTTC399000.017.6107758
CGTTACG40350.017.17415221
AGAACGA428100.017.14972144
AACGACG49200.016.82873716
TCAGACC454900.016.4862562
GCGTTCT453600.015.5752849
AATACGG463450.015.53106932
AAGAACG479150.015.31784543
TACGGAG462650.015.31503434
Produced by FastQC (version 0.11.5)