FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63547246
Sequences flagged as poor quality0
Sequence length46-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG42050.013.26979926
AACTCTA149900.012.47359745
AGCTTAG206800.012.22948445
ATAGGGG223050.011.72167738
GGCATAG217350.011.67690735
GAGCTTA237650.010.7271644
GGGAGGT339600.010.5942123
GGAGGTC329300.010.3857714
GTCACTC315000.010.3641858
CATAGGG247650.010.3028637
GGTCACT319250.010.1627847
ACCCGGC87800.010.0199775
ACTCTCC329200.09.97181311
TGGCATA258200.09.95149134
AGGTCAC347900.09.9467286
GCATAGG261450.09.72461936
ATATAGC67250.09.6690526
TGTCGTT62400.09.3388628
TAATCGG80650.09.3181344
GAGGTCA379400.09.3048665
Produced by FastQC (version 0.11.5)