FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81360500
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA692600.010.47063394-95
ACACGTC1001800.09.886044594-95
ACGTCTG839550.09.48992894-95
CACGTCT977000.09.17269794-95
CACACGT1203550.09.14451694-95
ATCGGAA3088250.08.69433694-95
GCACACG1425150.08.54486294-95
GATCGGA3385300.08.53870394-95
AGATCGG3783750.08.321177594-95
TCGGAAG2937650.08.29672394-95
TAGATCG1095500.08.24684594-95
CGGAAGA2768600.08.2151794-95
AGAGCAC2136450.07.85175694-95
AGCACAC1799050.07.698326694-95
GTCTGAA925600.07.564707394-95
GAGCACA2081750.07.44348294-95
CAGATCG1296550.07.369487394-95
AAGAGCA2573950.07.25938194-95
AAGATCG1179700.07.258931694-95
TCTGAAC842300.07.20122694-95
Produced by FastQC (version 0.11.5)