FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81923083
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA596700.010.10804794-95
ACACGTC875400.09.8145794-95
ACGTCTG742300.09.25563394-95
CACACGT1056700.09.14836494-95
ATCGGAA2755050.08.98928794-95
CACGTCT862650.08.94695794-95
GATCGGA3040800.08.92297294-95
GCACACG1276550.08.783380594-95
TCGGAAG2645600.08.75417394-95
AGATCGG3414050.08.60529794-95
CGGAAGA2495450.08.60387494-95
TAGATCG960400.08.59770394-95
AGAGCAC1931300.07.803048694-95
AGCACAC1607200.07.733385694-95
CAGATCG1279700.07.644292494-95
GAGCACA1894750.07.44534694-95
AAGATCG994700.07.391109594-95
AAGAGCA2354150.07.092200394-95
GTCTGAA830450.06.84975794-95
GAAGAGC2680200.06.83289794-95
Produced by FastQC (version 0.11.5)