FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73616339
Sequences flagged as poor quality0
Sequence length50-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1022530.13889987112779406No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT903590.12274313179306566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG97450.029.3464186
TCGACGA108050.026.6538057
CGGTATA153700.026.24704420
CGACGAG113200.025.242448
GTTCGAC115650.024.7866345
CAGTTCG138350.021.4353943
AGTTCGA137450.021.2482914
GTATAGA208500.020.50319722
ACGAGGA183800.020.43124810
ATAGAGT215800.019.76791824
ACCGCGT221200.019.754026
ATGACGG196200.019.5753716
GACCGCG225150.019.3775845
AGACCGC229150.019.1571754
CGAGGAT183150.019.0295511
GACGAGG205650.018.2057029
CCGCGTT239950.018.172187
TAGAGTT240150.017.96029725
CAGACCG251200.017.6547783
CCGGCTT363800.017.5837862
Produced by FastQC (version 0.11.5)