FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73616339
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1330530.18073840917299624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA213250.016.27134345
AGCTTAG398750.016.18476945
GGGAGGT567000.015.1973643
ATAGGGG434050.014.96332238
GGAGGTC558750.014.9307064
GGCATAG440900.014.62093435
TATATAC101250.014.576987
GTCACTC563250.014.5118948
GGTCACT584450.014.0124077
GAGCTTA467300.013.8979444
AGGTCAC605700.013.8253846
GAGGTCA615900.013.6694725
ATATATA114300.013.6606616
ACTCTCC614250.013.47181711
TGGCATA490200.013.28360134
GTTGTCG43250.012.79679626
CATAGGG511100.012.742187537
TCACTCT674750.012.2706069
CCATATA124350.012.15854554
GCATAGG539100.012.04947336
Produced by FastQC (version 0.11.5)