FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67730589
Sequences flagged as poor quality0
Sequence length34-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2311940.3413435545348646No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1220840.18024942910211514No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG884960.13065883717621296No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA838070.12373582045772553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG108900.030.3460886
TCGACGA116450.028.2244197
CGACGAG120550.026.8917458
CGGTATA167350.025.626720
GTTCGAC130150.025.3053785
AGTTCGA152600.022.0098824
CAGTTCG157300.021.9378243
ATGACGG185900.021.30519116
GACGAGG187050.021.16259
ACGAGGA192850.020.66718910
GAACGAT656150.020.54068245
GTATAGA231350.019.72208822
ACCGCGT669400.019.43176
AGACCGC682200.019.2356364
GACCGCG673850.019.1869145
CAGACCG689500.019.0775973
CGAGGAT192550.018.89688111
CCGCGTT691850.018.794767
CGCGTTC699500.018.6020458
GTTCCTA170800.018.52735315
Produced by FastQC (version 0.11.5)