FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75747165
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA575550.010.33561194-95
ACACGTC845150.09.68272994-95
ACGTCTG707100.09.62328594-95
CACGTCT825350.09.20854194-95
CACACGT1016850.09.02512294-95
ATCGGAA2679850.08.93054494-95
GATCGGA2952900.08.85083194-95
TCGGAAG2555950.08.81728894-95
AGATCGG3313750.08.711349594-95
GCACACG1218050.08.67804394-95
CGGAAGA2397350.08.62780894-95
TAGATCG955150.08.38417494-95
AGAGCAC1850900.07.936278394-95
CAGATCG1225800.07.913171394-95
AGCACAC1546800.07.522586394-95
TAGCGAC63650.07.5138742
AAGATCG980900.07.508140694-95
GAGCACA1790550.07.488304694-95
ATAGCGA75300.07.35755541
AAGAGCA2267500.07.207822394-95
Produced by FastQC (version 0.11.5)