FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80596274
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA797600.010.64211894-95
ACACGTC1181600.010.03847894-95
ACGTCTG980800.09.97057494-95
CACGTCT1135600.09.38203394-95
CACACGT1399400.09.17574394-95
ATCGGAA3628900.08.65501794-95
TCGGAAG3458850.08.50939694-95
GATCGGA3996550.08.45212294-95
CGGAAGA3264250.08.4159394-95
GCACACG1704750.08.40469994-95
GTCTGAA956750.08.37076494-95
AGAGCAC2447300.08.23134294-95
AGATCGG4444100.08.10524194-95
TCTGAAC870800.08.08922794-95
AGCACAC2025250.07.91600894-95
TAGATCG1136050.07.873311594-95
GAGCACA2334000.07.816704894-95
CAGATCG1720750.07.697149394-95
AAGAGCA2868500.07.62577794-95
GAAGAGC3303400.07.23994594-95
Produced by FastQC (version 0.11.5)