FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78640961
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1218600.09.52158494-95
GATCGGA1346000.09.4235594-95
AGATCGG1547000.09.13716694-95
TAGATCG464650.09.09524594-95
TCGGAAG1239700.08.56207694-95
CGTCTGA316200.08.02648994-95
CGGAAGA1217500.07.967938494-95
ACACGTC439900.07.852481494-95
CAGATCG645200.07.3789194-95
AAGATCG536100.07.18749194-95
CACACGT557050.07.119556494-95
ACGTCTG406900.06.898020794-95
CACGTCT474250.06.619352394-95
GCACACG686500.06.4802494-95
GAGATCG600700.05.971825694-95
AGAGCAC1122750.05.839195394-95
AGCACAC975300.05.56016494-95
GAAGAGC1600350.05.4530794-95
AAGAGCA1429400.05.433840894-95
GAGCACA1158800.05.418066594-95
Produced by FastQC (version 0.11.5)