FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79583887
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA572350.010.16439394-95
ACACGTC841000.09.49036594-95
ACGTCTG717100.09.42494694-95
ATCGGAA2588450.09.06076694-95
GATCGGA2865000.08.87551994-95
CACACGT1017150.08.80626294-95
CACGTCT834950.08.75558194-95
TCGGAAG2508750.08.70723894-95
AGATCGG3220000.08.63123494-95
CGGAAGA2377400.08.5163894-95
GCACACG1241600.08.25294894-95
TAGATCG874650.08.2153894-95
AGAGCAC1859250.07.665499794-95
CAGATCG1234200.07.634094794-95
AAGATCG929400.07.393912394-95
AGCACAC1570100.07.332778594-95
AAGAGCA2218650.07.135913494-95
GAGCACA1822550.07.05492394-95
GTCTGAA785900.06.97006294-95
GAAGAGC2551800.06.91565594-95
Produced by FastQC (version 0.11.5)