FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78922388
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA556550.010.10869894-95
ACACGTC826000.09.51938694-95
ACGTCTG697600.09.27825894-95
ATCGGAA2441600.09.03818694-95
CACACGT1007000.08.87142394-95
CACGTCT821700.08.85053794-95
GATCGGA2691150.08.675693594-95
TCGGAAG2366650.08.47629794-95
AGATCGG3022250.08.45675694-95
TAGATCG849500.08.27325694-95
CGGAAGA2256850.08.05730494-95
GCACACG1229450.08.04235594-95
CAGATCG1125050.07.511336394-95
AGAGCAC1804950.07.423380494-95
AAGATCG925450.07.308242394-95
AGCACAC1518400.07.1977794-95
AAGAGCA2149700.07.11517694-95
GTCTGAA756350.07.069124794-95
GAGCACA1768250.06.863467794-95
TCTGAAC699900.06.766457694-95
Produced by FastQC (version 0.11.5)