FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55497543
Sequences flagged as poor quality0
Sequence length34-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1082910.1951275572686164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT265550.021.80634745
AGACCGC259150.021.5049424
GACCGCG256650.021.459925
ACCGCGT260100.021.3131566
CCGCGTT271300.020.4664977
CAGACCG281100.020.041813
CGCGTTC279400.019.873168
TCAGACC326250.018.2634722
AGAACGA328250.017.65325444
GCGTTCT324000.017.2555949
GTTCCTA116550.016.77468915
AATACGG346200.016.65689332
ATACGGA347600.016.6027533
CTCAGAC400850.016.559821
TACGGAG345050.016.42659434
TTCGACG20650.016.1246056
CAATACG363600.016.02059631
AAGAACG367200.015.8112243
CCTACTC126500.015.4552318
CGTTACG30800.015.41378421
Produced by FastQC (version 0.11.5)