FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55497543
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG195450.012.96753345
GGCATAG195650.012.94936335
ATAGGGG203500.012.7708238
TCAGACG89050.012.2814852
GGGAGGT304750.012.1762123
GGAGGTC298950.012.0210834
GTCACTC288550.011.780388
GGTCACT298800.011.4141997
TGGCATA226800.011.27974934
ACTCTCC309350.011.08270211
GAGCTTA229700.011.07281444
GTTGTCG37550.011.02469226
AGGTCAC324700.010.9402366
CATAGGG231450.010.86886637
GAGGTCA335250.010.8383385
GCATAGG247100.010.3261336
CAGACGT110650.09.7820283
ATATAGC50750.09.7081166
CGTGGCG155350.09.64742851
TCACTCT363450.09.3959999
Produced by FastQC (version 0.11.5)