FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34300184
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC30350.014.9823096
TCGTATG23650.012.57105645
TTCCGAT38050.012.0423365
CGTATGC26900.011.57235446
CTCGTAT26850.010.81267244
TAGATCG51350.010.76291170
TATGCCG27450.010.32119148
CCGATCT46100.010.2429947
CGATCTA16250.09.2556998
AGATCGG151250.08.97324370
GATCGGA123500.08.89356370
ATCGGAA111550.08.65471270
TCTCGTA37750.07.597943
CAGATCG92200.06.10812770
TCGGAAG149450.05.570570
GTATGCC59900.05.488896447
AAGATCG96450.05.29495769
CTTCCGA102750.05.24228144
ATCTCGT60450.05.149761742
CGGAAGA154950.05.12445270
Produced by FastQC (version 0.11.5)